NEW STEP BY STEP MAP FOR ML162

Finally, one μCi of 86Rb was additional to every tradition and incubation was ongoing at 37 °C for yet another 10 min. Reactions have been terminated by washing the cells with ice-chilly 100 mM MgCl2 and cell lysates had been organized for liquid scintillation counting. The 86Rb counts in parallel cultures with ouabain were being subtract